Molecular Biology & Genetic Engineering

(Pre-requisites: MCB 201 & MCB 202)

Overview of DNA replication. Review of gene expression (transcription, translation and regulation). Principles of recombinant DNA technology: restriction enzymes, vectors and creation of DNA libraries. Molecular techniques for studying gene expression: Southern, northern and western blotting. Microorganisms as tools: Microbial enzymes, transformation, cloning and expression techniques. Human gene cloning, stem cell technologies and tissue engineering. DNA fingerprinting and forensic analysis. Genomics and bioinformatics. Ethical considerations in genetic engineering. Polymerase chain reaction (PCR); Gene Editing, Gene Sequencing, Restriction fragment length polymorphism (RFLP); Pulsed Field Gel Electrophoresis (PFGE); Nucleic Acid Hybridization –Solution phase hybridization, Solid phase hybridization, Dot hybridization, Sandwich hybridization, Southern blot hybridization, Northern blot hybridization, Clinical applications of hybridization techniques.