Microbial Genetics

(Pre-requisites: MCB 102 & either MCB 201 or MCB 202)

Concept and scope of microbial genetics. DNA as genetic material and molecular basis of inheritance. A review of the chemistry of DNA and RNA. Gene expression: transcription; translation and regulation (examples with the LAC operon, TRP operon, Lambda lytic and lysogenic cycles). Mutation and mutagenesis. Types of mutants and their use in scientific studies. Genetic interaction amongst microorganisms: Recombination; complementation; conjugation; transduction; transformation; insertional sequences.