Industrial Microbiology & Biotechnology I

(Pre-requisites: MCB 201 & MCB 202)

Definition, scope & nature of industrial microorganisms & biotechnology. Microorganisms of industrial importance and their major characteristics. Sources, isolation and screening for industrial microorganisms. Strains development, culture collection and maintenance. Formulation and preparation of media for industrial cultivation of microorganisms. Bioreactors for cultivation of microorganisms – design and operation. Sterilization of bioreactors, media and gasses. Mass transfer in bioreactors. Cell cultivation – culture systems (batch, repeated batch, fed-batch and continuous cultivation of microorganisms). Kinetics of microbial growth, substrate utilization and product formation in batch cultures. Bio-process monitoring and control. Scale-up of fermentation processes.