General Biochemistry Practical

Introduction to laboratory and laboratory equipment. Safety, housekeeping, washing and drying of glassware in the laboratory. Accuracy of measurement and transfer of liquids and solids. Introduction to photometry and colorimetry; standard curve and absorption spectra; pH and buffer systems. Qualitative and quantitative tests for amino acids and proteins. Biuret method and the estimation of proteins. Reaction of carbohydrates; thin layer chromatographic separation of sugars. Estimation of glucose in biological fluids (blood and urine). Analysis of lipid for double bonds and free fatty acids; separation by thin layer chromatography. Separation and purification of nucleic acids; estimation of DNA and RNA and estimation of phosphate sand titratable acidity.