Cell and Molecular Biology Techniques

Safety in molecular biology laboratory. Media, sterilisation, explants, micro propagation, callus culture, organogenesis, embryogenesis, somatic variation, double haploids, interspecific hybrids, protoplast fusion and environmental conditions required for these processes. Animal, microbial and plant cell and tissue culture theory and practice. The role of tissue culture in modifying plants in plant biotechnology to the propagation of endangered plants and from modifying cell lines in biotechnology to the propagation of cell lines for use in medical, microbiological, and biochemical research and applications. Upon completion of the course, students will acquire the basic knowledge and skills needed to perform cloning experiments, PCR and DNA sequencing techniques, as well as protein expression techniques.