Advanced Enzymology

Chemistry of enzyme catalysis. Steady state enzyme kinetics. Transport kinetic methods. Ligand binding and its application to enzymology. Kinetics of multiple binding sites., mechanisms of two substrate systems. Molecular models of allosterism. Enzyme models of allosterism. Multi-enzyme complexes. Enzyme assays and techniques in enzyme reconstitution. Regulation of enzyme activity and synthesis. Chemical basis of enzyme catalysis. Enzyme reaction mechanisms, eg ribonuclease, chymotrypsin, lysozyme, LDH, etc. Site directed mutagenesis. Pre-requisite: BCH 301