Pharmaceutical Biotechnology

Basic techniques in biotechnology-cutting and joining of DNA molecules. Cloning techniques. Construction of RNA structure. Screening methods. DNA analysis. Mutagensis. Polymerase Chain Reaction (PCR). Clinical importance of recombinant proteins e.g. human insulin, growth hormones, interferon. Biotechnology in vaccine development-DNA vaccines, vaccine production by recombinant DNA for prevention of viral and bacterial infections. Identification of potential biotechnological products; plants and transgenic animals as potential sources of recombinant biotechnological products. Characterization of expressed proteins. An overview of biotechnological products in current use.