Analytical Microbiology & Quality Control

(Pre-requisites: MCB 201 & MCB 202)

General principles of analytical microbiology, concept of microbiological analysis, concept of microbial analysis. Microbiological analytical techniques (qualitative microbiological analytical techniques, quantitative microbiological techniques, culture methods for enumeration of microorganisms including basic principles, pour plate method, spread plate method, most probable number techniques, membrane filtration techniques, anaerobic counts. Microscopic methods (fluorescent microscopy, stainings & probes). Quantitative microscopic analysis (haemocytometers, dried film method etc). Non cultural methods for microbial enumeration (use of cell wall & cytoplasmic components). Immunological & serological analytical techniques serological analysis, immuno-magnetic techniques, enzyme linked immuno-absorbent assay (ELIZA) techniques). Instrumental techniques (spectrophotometry, centrifugation, chromatography, electrophoresis manometric etc). Enzymatic techniques (principles, applications, biosensors). Molecular analytical techniques (general principles, molecular typing & differentiation, multilocus enzyme electrophoresis, ribotyping, restriction fragment length polymorphism, cellular fatty acid analysis, rapid PCR methods).