Fundamental of Blood Group Serology

Techniques – Principles, Disadvantages and Advantages. Preparation of Antisera – antiserum titration, avidity, potency and specificity. Plant lectins – Preparation and Standardization of antisera from lectins e.g. Dolichos biflorus Anticoagulants used in BGS, ACD, CPD-CPA-A etc. Modes of Action, Side effects. Blood Bottles (MRC) and Plastic Bags – Advantages and disadvantages. Donor Screening – using Cuso4 method – other methods of screening. Preparation of blood products – cryoprecipitate, platelet rich plasma, packed cell fresh frozen plasma, fibrinogen etc.

Storage of blood and blood products – various methods, advantages and disadvantages. Blood banking-organization, structures, facilities and records. Blood group specific substances – synthesis, identification method(s) and application. Quality control of physical, chemical and reagent. Practical/tutorials ABO and Rhesus grouping methods, Antiserum Titration DCT and ICT antibody screening.